Wu Laboratory

Selected Publications

Dykstra H, Fisk C, LaRose C, Waldhart A, Meng X, Zhao G, Wu N. 2021. Mouse long-chain acyl-CoA synthetase 1 is active as a monomer. Arch Biochem Biophys 700:108773.

Waldhart AN, Muhire B, Johnson B, Pettinga D, Madaj ZB, Wolfrum E, Dysktra H, Wegert V, Pospisilik JA, Han X, Wu N. 2021. Excess dietary carbohydrate affects mitochondrial integrity as observed in brown adipose tissue. Cell Rep 35(5):109488.

Lee HJ, Jedrychowski MP, Vinayagam A, Wu N, Shyh-Chang N, Hu Y, Min-Wen C, Moore JK, Asara JM, Lyssiotis CA, Perrimon N, Gygi SP, Cantley LC, Kirschner MW. 2017. Proteomic and metabolic characterization of a mammalian cellular transition from quiescence to proliferation. Cell Rep 20(3):721–736.

Waldhart AN, Dykstra H, Peck AS, Boguslawski EA, Madaj EA, Wen J, Veldkamp K, Hollowell M, Zheng B, Cantley LC, McGraw TE, Wu N. 2017. Phosphorylation of TXNIP by AKT mediates acute influx of glucose in response to insulin. Cell Rep 19(10):2005–2013.

Wu N, Zheng B, Shaywitz A, Dagon Y, Tower C, Bellinger G, Shen C, Wen J, Asara JM, McGraw TE, Kahn BB, Cantley LC. 2013. AMPK-dependent degradation of TXNIP upon energy stress leads to enhanced glucose uptake via GLUT1. Mol Cell 49(6):1167–1175.         

Christofk HR, Wu N, Cantley LC, Asara JM. 2011. Proteomic screening method for phosphopeptide motif binding proteins using peptide libraries. J Proteome Res 10(9):4158–4164.

Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC. 2008. Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature 452:181–186.

Asara JM, Zhang X, Zheng B, Maroney LA, Christofk HR, Wu N, Cantley LC. 2006. In-gel stable isotope labeling for relative quantification using mass spectrometry. Nat Protoc 1(1):46–51.

Asara JM, Zhang X, Zheng B, Christofk HH, Wu N, Cantley LC. 2006. In-gel stable-isotope labeling (ISIL): a strategy for mass spectrometry-based relative quantification. J Proteome Res 5(1):155–163.

Summerer D, Chen S, Wu N, Deiters A, Chin JW, Schultz PG. 2006. A genetically encoded fluorescent amino acid. Proc Natl Acad Sci U.S.A. 103(26):9785-9789.

*Benes CH, *Wu N, Elia AE, Dharia T, Cantley LC, Soltoff SP. 2005. The C2 domain of PKCd is a phosphotyrosine binding domain. Cell 121(2):271–280.

Xie J, Wang L, Wu N, Brock A, Spraggon G, Schultz PG. 2004. The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination. Nat Biotechnol 22:1297–1301.

Garces RG, Wu N, Gillon W, Pai EF. 2004. Anabaena circadian clock proteins KaiA and KaiB reveal a potential common binding site to their partner KaiC. EMBO J 23:1688–1698.

Wu N, Deiters A, Cropp TA, King D, Schultz PG. 2004. A genetically encoded photocaged amino acid. J Am Chem Soc 126(44):14306–14307.

*Anderson JC, *Wu N, Santoro SW, Lakshman V, King DS, Schultz PG. 2004. An expanded genetic code with a functional quadruplet codon. Proc Natl Acad Sci U. S. A. 101(20):7566–7571. 

Wu N, Pai EF. 2004. Crystallographic studies of native and mutant orotidine 5’-phosphate decarboxylase. Topics Curr Chem 238:23–42.

Wu N, Pai EF. 2002. Crystal structures of inhibitor complexes reveal alternate binding mode in orotidine 5’-monophosphate decarboxylase. J Biol Chem 277(31):28080–28087.

Wu N, Gillon W, Pai EF. 2002. Mapping the active site-ligand interactions of Orotidine 5’-monophosphate decarboxylase by crystallography. Biochemistry 41(12):4002–4011.

Wu N, Mo Y, Gao J, Pai EF. 2000. Electrostatic stress in catalysis: structure and mechanism of the most proficient enzyme orotidine monophosphate decarboxylase. Proc Natl Acad Sci U.S.A. 97(5) 2017–2022.

Wu N, Christendat D, Dharamsi A, Pai EF. 2000. Purification, crystallization and preliminary X-ray study of orotidine 5’-monophosphate decarboxylase. Acta Crystallographica D56(7):912–914.

Christendat D, Yee A, Dharamsi A, Kluger Y, Savchenko A, Cort JR, Booth V, Mackereth CD, Saridakis V, Ekiel I, Kozlov G, Maxwell KL, Wu N, McIntosh LP, Gehring K, Kennedy MA, Davidson AR, Pai EF, Gerstein M, Edwards AM, Arrowsmith CH. 2000. Structural proteomics of an archaeon. Nat Struct Biol 7:903–909